rabbit anti neuronal nuclei antigen  (Proteintech)

Journal: Journal of Neuroinflammation

Article Title: Conditional knockout of Tsc1 in RORγt-expressing cells induces brain damage and early death in mice

doi: 10.1186/s12974-021-02153-8

Figure Lengend Snippet: Tsc1 RORγt mice exhibited spontaneous tonic-clonic seizures with neuronal defects. a Representative images of spontaneous generalized tonic-clonic seizures at different phases observed in the Tsc1 RORγt mice. These images were extracted from a video of Tsc1 RORγt mice housed with control littermates. b The brain weights of Tsc1 RORγt mice and control littermates. c Representative images of HE staining and thickness of the cortex of the brains of Tsc1 RORγt mice and control littermates. d, e Representative HE staining images of the cortex ( d ) and hippocampus ( e ) of Tsc1 RORγt mice and control littermates. f Representative images of NeuN immunostaining (red) and the cell scattering width in the CA and DG regions of the hippocampus from Tsc1 RORγt mice and control littermates. Each dot represents one mouse, and error bars represent SEMs; * p < 0.05, ** p < 0.01. Unpaired two-tailed Student’s t test ( b , c , f ). All images were captured from scans of whole-brain slices. Magnification: × 10 ( c ), × 20 (upper panels in d , e ), and × 40 (lower panels in d, e , f ). Scale bars: 50 μm ( d , e , f ) and 100 μm ( c )

Article Snippet: The brain sections were incubated with a polyclonal rat anti-mouse glial fibrillary acidic protein (GFAP) antibody (eBioscience) and/or polyclonal rabbit anti-mouse neuronal nuclei antigen (NeuN) antibody (eBioscience) in 10% goat serum overnight at 4 °C after deparaffinization, rehydration, and antigen retrieval.

Techniques: Control, Staining, Immunostaining, Two Tailed Test

Journal: Journal of Neuroinflammation

Article Title: Conditional knockout of Tsc1 in RORγt-expressing cells induces brain damage and early death in mice

doi: 10.1186/s12974-021-02153-8

Figure Lengend Snippet: Abnormal activation of neurons and astrogliosis in the cortex and hippocampus of Ts c1 RORγt mice. a – d Representative images of Nissl staining of the cerebral cortex ( a ) and different regions of the hippocampus ( b – d ) of Tsc1 RORγt mice and control littermates. e Statistical analysis of the area fraction of Nissl bodies shown in a – d . f – h Representative images of GFAP immunostaining (green) ( f and j ) and the area fraction of GFAP + astrocytes and NeuN + neurons ( h ) in the cerebral cortex ( f ) and hippocampus (b) of Tsc1 RORγt mice and control littermates. Each dot represents one mouse and the error bars represent SEMs; * p < 0.05 and ** p < 0.01. Unpaired two-tailed Student’s t test ( e , f , g ). The locations of the magnified box are identified in the original image. All images were captured from scans of whole-brain slices. Magnification: 10x ( a , f ) and × 40 ( b , c , d , g ). Scale bars: 50 μm ( b , c , d , g ) and 100 μm ( a , f )

Article Snippet: The brain sections were incubated with a polyclonal rat anti-mouse glial fibrillary acidic protein (GFAP) antibody (eBioscience) and/or polyclonal rabbit anti-mouse neuronal nuclei antigen (NeuN) antibody (eBioscience) in 10% goat serum overnight at 4 °C after deparaffinization, rehydration, and antigen retrieval.

Techniques: Activation Assay, Staining, Control, Immunostaining, Two Tailed Test